Two distinct proton translocating ATPases are present in membrane vesicles from radish seedlings
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چکیده
منابع مشابه
Fluoride inhibition of proton-translocating ATPases of oral bacteria.
The ATPases of isolated membranes of lactic acid bacteria were found to be inhibited by fluoride in a complex manner. Among the enzymes tested, that of Streptococcus mutans GS-5 was the most sensitive to fluoride, and the initial rate of hydrolysis of ATP was reduced 50% by approximately 3 mM fluoride. The enzyme of Lactobacillus casei ATCC 4646 was the most resistant, and about 25 mM fluoride ...
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Polysaccharide-containing vesicles were collected from secretory cells maintained in liquid culture. Characterization of membrane-associated nucleosidephosphatases revealed that the vesicles specifically hydrolyze ATP, have a pH optimum between 6.0 and 6.5, and are stimulated by inorganic cations, especially K(+). The ATPase activity in these vesicles was inhibited by orthovanadate and N,N'-dic...
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Pea root microsomal vesicles have been fractionated on a Dextran step gradient to give three fractions, each of which carries out ATP-dependent proton accumulation as measured by fluorescence quenching of quinacrine. The fraction at the 4/6% Dextran interface is enriched in plasma membrane, as determined by UDPG sterol glucosyltransferase and vanadate-inhibited ATPase. The vanadate-sensitive ph...
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arriving at (c ,+c ,+P) in the absence of antimycin must represent the oxidation of a second equivalent of QH, by the cyt. cI which remained oxidized after the first cycle of the complex. This second electron transfer presumably also occurs via the FeS centre, so that effectively the ‘invisible’ electron on the FeS centre gets replaced and pushed over on to cyt. c, in a second turnover of the c...
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Proton-coupled lactate transport across the basolateral membrane of rat jejunal enterocyte was studied using well purified membrane vesicles. L-lactate uptake is stimulated by an inwardly directed H+ gradient; the effect of the pH difference is drastically reduced by FCCP and by pCMBS; unlabelled L-lactate causes a strong inhibition, whilst furosemide is uneffective. The H+ gradient-dependent s...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1983
ISSN: 0014-5793
DOI: 10.1016/0014-5793(83)81054-0